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现在位置:首页 > 科研进展 > 最新重要论文(影响因子PNAS及以上)
Molecular resolution imaging by repetitive optical selective exposure,Nat Methods, 09 Sep 2019
2019-09-09 | 【     】【打印】【关闭

Nature Methods09 September, 2019,DOI:

Molecular resolution imaging by repetitive optical selective exposure

Lusheng Gu, Yuanyuan Li, Shuwen Zhang, Yanhong Xue, Weixing Li, Dong Li, Tao Xu & Wei Ji

Abstract

We introduce an interferometric single-molecule localization method for super-resolution fluorescence microscopy. Fluorescence molecules are located by the intensities of multiple excitation patterns of an interference fringe, providing around a twofold improvement in the localization precision compared with the conventional imaging with the same photon budget. We demonstrate this technique by resolving nanostructures down to 5 nm in size over a large 25 × 25 μm2 field of view.

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